Ultrastructure of foot coronary corium in Nelore cattle

Franco da Silva LA, Belett ME, Eurides D, Rabelo RE, Ribeiro de Freitas SL, Bastos Queiroz PJ, Alves da Silva J., Ultrastructure of foot coronary corium in Nelore cattle, Onl J Vet Res., Nelore cattle are known to be rustic and have greater resistance to foot diseases. Nevertheless, their occurrence, even at extensive farming system, is still observed. The ultrastructural examination shows up as an alternative to identify the pathophysiology of most foot problems. This study aimed to identify the coronary band structures of Nelore cattle hooves by means of transmission electron microscopy (TEM) and describe some difficulties encountered during samples’ processing. Fifteen Nelore cattle, managed under intensive grazing, were used in the present study, of which three samples from the coronary band measuring 1mm were collected after slaughter. Samples were evaluated using a transmission electron microscope and imaged using Megaview GII Image Capture system/Olympus Soft Imaging Solutions. Ultrastructural analysis of the coronary band was descriptive and allowed identification of keratinocytes and desmosomes tonofilaments. The long time frame between collecting and analyzing tissue samples were associated with dryness, which interfered with material quality, as well as some issues encountered during samples’ process.

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